Rapid detection of Candida parapsilosis contamination in the infusion fluid

Abstract

We encountered a case of microbial contamination of infusion fluid detected based on the discoloration of an inline filter (membrane filter) in the main route. A cause of this contamination may be the use of an extension tube connected to the main route for more than a month. In the infusion fluid above the inline filter (on the high calorie end), 2.4 × 10⁵ colony forming units (cfu)/ml of Candida parapsilosis was detected. “Adenosine triphosphate (ATP) + adenosine monophosphate (AMP)” was determined in addition. We conclude that microbial contamination of this infusion fluid can be rapidly estimated by measuring the amounts of “ATP + AMP”.